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Accredited methods description 17-06-2020

                                                                                                                                                             

Reference number

Lab-code

Analyses

Matrix

 Units

M003

Ak30_t

Aerobic plate count 30°C in food

In accordance with ISO 4833-1:2013

Food

Enumeration cfu/gram

M069

Ak30pf_t

Aerobic plate count 30°C petrifilm in food

Equivalent to ISO 4833-1:2013

Instead of growth medium PCA, petrifilm (3M) is used. Validated by Afnor 3M 01/1-09/89 and internally 

Food

Enumeration cfu/gram

M001

Aes_t

Aerobic spore formers

Own method

After heating the product 10 minutes at 80°C, growth at TSA medium after 24 hours at 37°C is counted.

Method commonly used and described in “Microbiologie van Voedingsmiddelen”, ISBN 978-90-8572-056-0. Validated internally

Food

Enumeration cfu/gram

M007

Ans_t

Anaerobic spore formers

After heating the product 10 minutes at 80°C, anaerobic growth at SA medium after 48 hours at 37°C is counted.

Method commonly used and described in “Microbiologie van Voedings-middelen”, ISBN 978-90-8572-056-0. Validated internally.

Food

Enumeration cfu/gram

M011

Bc_t

Bacillus cereus

In accordance with ISO 7932:2004

Food

Enumeration cfu/gram

M021

Cf_t

Coliforms in foods

In accordance with ISO 4832:2006

Food

Enumeration cfu/gram

M027

Ec_t

E. coli

In accordance with ISO 16649-2:2001

Food

Enumeration cfu/gram

M077

EcH7_g

E. coli O157:H7 in meat or vegetables

In accordance with AOAC 996.09:2012

Food

Detection in 25 gram

M023

En_t

Enterobacteriaceae in food

In accordance with ISO 21528-2:2004 without confirmation

Food

Enumeration cfu/gram

M116

Gdroog_t

Sdroog_t

GSdroog_t

Yeasts and/or moulds in products with Water activity ≤0,95

In accordance with ISO 21527-2:2008

Food

Enumeration cfu/gram

M117

Gnat_t

Snat_t

GSnat_t

Yeasts and/or moulds in products with Water activity >0,95

In accordance with ISO 21527-1:2008

Food

Enumeration cfu/gram

M032

Lb_t

Lactobacilli enumeration in dairy products

In accordance with NEN 6815:2009

Food

Enumeration cfu/gram

M033

Lm_g 

Listeria monocytogenes in food and environmental samples

In accordance with ISO 11290-1:2017

Validated internally for swabs

Food and environmental samples

Detection in 25 gram

Lmswab_g

Detection in swab

M033a

Lm200_fg

Listeria monocytogenes in food

In accordance with ISO 11290-1:2017

Validated internally for filtrated samples.

Food

Detection in 200 ml

M033b

LmR_g

Listeria monocytogenes in food and environmental samples

Equivalent to ISO 11290-1:2017

Food and environmental samples

Detection in 25 gram

LmRswab_g

Detection in swab

M109

LmPCR_g

Listeria monocytogenes in food and environmental samples

Equivalent to ISO 11290-1:2017

Validated internally for swabs

After 24 hours incubation in 24 LEB broth at 37°C, the presence of DNA is detected by PCR. Validated by AFNOR UNI 03/08-11/13 and internally

Food and environmental samples

Detection in 25 gram

LmPCRswab_g

Detection in swab

M109a

LmPCR200_fg

Listeria monocytogenes in food

Equivalent to ISO 11290-1:2017 Validated internally for filtrated samples.

After 24 hours incubation in 24 LEB broth at 37°C, the presence of DNA is detected by PCR. Validated by AFNOR UNI 03/08-11/13 and internally

Food

Detection in 200 ml

M035

Lm100_t 

Listeria monocytogenes enumeration < 100 cfu/g

In accordance with ISO 11290-2:2017

Food

Enumeration cfu/gram

Lm10_t 

Listeria monocytogenes enumeration < 10 cfu/g

In accordance with ISO 11290-2:2017

Lm1_t

Listeria monocytogenes enumeration < 1 cfu/g

In accordance with ISO 11290-2:2017

M036

Mzb_t

Lactic acid bacteria in food

In accordance with ISO 15214:1998, including confirmation

Food

Enumeration cfu/gram

M047

Salm_g

Salmonella spp. detection in food

Equivalent to ISO 6579:2017

After 20 hours incubation in One Broth Salmonella at 42°C, the growth is observed at a Brillliance growth medium after 24 hours at 37° C.

Validated by AFNOR UNI 03/06-12/07 and internally

Food

Detection in 25 gram

 

 

Salm10_g

Detection in 10 gram

M047b

Salm200_fg

Salmonella spp. detection in food

Equivalent to ISO 6579:2017

Validated internally for filtrated samples.

After 20 hours incubation in One Broth Salmonella at 42°C, the growth is observed at a Brillliance growth medium after 24 hours at 37° C.

Validated by AFNOR UNI 03/06-12/07 and internally

Food

Detection in 200 ml

M108

SalmPCR_g

Salmonella spp. detection in food

Equivalent to  ISO 6579:2017  

After 22 hours incubation in BPW at 37°C, the presence of DNA is detected by PCR. Validated by AFNOR UNI 03/07-11/13 and internally

Food

Detection in 25 gram

M067

STEC25_g

Shiga-toxin producing Escherichia coli (STEC) screening on Stx and eae genes

In accordance with ISO/TS 13136:2012

Validated internally for environmental samples and filtrated samples

Food and environmental samples

Detection in 25 gram

STECswab_g

Detection in swab

STEC125_g

Detection in 125 gram

STEC454_g

Detection in 454 gram

M067c

STEC100_fg

Detection in 100 ml

 

STEC200_fg

Detection in 200 ml

M068

STECbev

STECbev_rein

Shiga-toxin producing Escherichia coli (STEC) confirmation

In accordance with ISO/TS 13136:2012

Food

Confirmation procedure on Stx and eae genes

M083

STECsero

Serotyping of isolates by RT-PCR O26, O45, O103, O111, O121, O145, O157

In accordance with ISO/TS 13136:2012

Food

Serotypering isolates

M054

Sac100_t

Sac10_t

Staphylococcus aureus

In accordance with ISO 6888-2:1999

Food

Enumeration cfu/gram

M017

SredS_t

Spores of Sulphite-reducing bacteria (anaerobic)

In accordance with ISO 15213:2003

Food

Enumeration cfu/gram

SredB_t

Sulphite-reducing bacteria (anaerobic)

In accordance with ISO 15213:2003

M061

Water

Escherichia coli and coliforms

In accordance with ISO 9308-1:2014

Water (process water)

Detection in 100 ml

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